Conjugation
Part:BBa_K5170001:Design
Designed by: Subhanan Banerjee, Suvam Saswat Das, Avani, Anurag Sarkar, Parth Kumar Group: iGEM24_IISc-Bengaluru (2024-10-01)
A novel PROTAC using BBa_K5170002 against 231pTau
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 43
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the incorporation of the modified base in the binding region could interfere with the activity and affinity of the Aptamer as binders, we had to plan for terminal PCR or rather a poly C tailing.
Source
The Aptamer was obtained by rounds of SELEX in the literature. The Aptamer is available at BBa_K5170002.
References
1. Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W. (2018). Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein. Journal of the American Chemical Society, 140(43), 14314–14323. https://doi.org/10.1021/jacs.8b08645