Conjugation

Part:BBa_K5170001:Design

Designed by: Subhanan Banerjee, Suvam Saswat Das, Avani, Anurag Sarkar, Parth Kumar   Group: iGEM24_IISc-Bengaluru   (2024-10-01)


A novel PROTAC using BBa_K5170002 against 231pTau


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the incorporation of the modified base in the binding region could interfere with the activity and affinity of the Aptamer as binders, we had to plan for terminal PCR or rather a poly C tailing.


Source

The Aptamer was obtained by rounds of SELEX in the literature. The Aptamer is available at BBa_K5170002.

References

1. Teng, I-Ting., Li, X., Yadikar, H. A., Yang, Z., Li, L., Lyu, Y., Pan, X., Wang, K. K., & Tan, W. (2018). Identification and Characterization of DNA Aptamers Specific for Phosphorylation Epitopes of Tau Protein. Journal of the American Chemical Society, 140(43), 14314–14323. https://doi.org/10.1021/jacs.8b08645